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University of Kentucky Green House Facilities

Each organization comprising the TĪNĒ consortium has outstanding facilities available for conducting state of the art research on MNM, far too extensive to describe herein. Facilities for plant studies including extensive glasshouse space and full climate and light controlled growth chambers for laboratory and mesocosm exposures and facilities for aeroponic and hydroponic plant cultivation are found at Rothamsted Research (RR),the Lancaster Environment Centre (LEC), and UKY. RR houses facilities for bacterial culture, including temperature controlled incubators. Both the NERC-CEH and the Environmental Chemistry and Toxicology lab (ECTL) at UKY house facilities for exposure of both aquatic organisms and soil invertebrates. The facilities at CEH and UKY include animal care laboratories equipped with environmental growth chambers. Standard laboratory facilities, including fume hoods, laminar flow clean hoods, -20°C and -80°C storage, incubators, centrifuges, drying ovens, muffle furnaces, microwave digestion systems and water purification systems, etc. are available at all locations. Modern well equipped molecular biology facilities which include clean rooms for DNA/RNA preparation, thermocyclers, gel and microarray imaging systems, RT-PCR, and setups for protein and DNA electrophoresis are in the labs of PIs at UKY, CMU, Duke, RR, and CEH. CEH, UKY, and RR have dedicated microarray and bioinformatics support facilities to enable the analysis of gene expression data. The Rothamsted Center for BioImaging, created in partnership with Jeol-Gatan provides state-of-the-science electron, confocal and light microscopy, while UKY has core facilities of relevance to the project including, a mass spectrometry facility, two electron microscopy facilities, three laser confocal microscopy facilities, a microarray facility, a DNA sequencing facility, and an x-ray laboratory equipped with XRD and XRF. Extensive materials characterization facilities including, BET surface area and high resolution thermal analyzers are available at CEINT and through the UKY Center for Applied Energy Research. CU houses a 120 m2 pilot testing facility at their own sewage treatment work capable of operating a series of pilot plants treating up to 30m3/day organized into a series of pilot bays each supplied with sources of sewage, water, air, and electricity. The pilot facilities are coupled to laboratory that house both water chemistry and nanotechnology analytical tools including a LC/MS/MS coupled to a Waters Quattro Premier XE triple quadruple mass spectrometer fitted with a Z spray ESI source, ICP-MS, AA, GC-MS, GC-ECD as well as biomass and soil respirometer.

Many laboratories in the TĪNĒ consortium have modern environmental analytical chemistry laboratories for automated analysis of major ions (Ion chromatography), carbon analyzers, HPLCs , and FT-IRs. The LEC houses a specialty clean room suite for trace metal work, with rooms specified to class 100 and class 1000 standards. Hydrogels and DGT components are routinely fabricated and assembled within the clean room environment. Several ICP-MS instruments are in laboratories of the TĪNĒ consortium members. The ECTL at UKY houses an Agilent 7500cx octopole collision cell ICP-MS that is routinely coupled to a variety of peripheral devices, including a Wyatt Eclipse III AF4 fractionation system. The AF4 is equipped with multiple detectors including a 18 angle laser light scattering detector with both static and dynamic light scattering capabilities, a diode array refractive index detector, a diode array UV-VIS detector, and a 3-D fluorescence detector. Other peripherals include a capillary zone electrophoresis system with UV-VIS diode array and interface for ICP-MS detection. For spatial and solid sample trace element analysis, both the ECTL at UKY and the LEC have laser ablation systems coupled to ICP-MS. Facilities housing devices for saturated transport experiments are located at ECTL UKY, at Duke and CMU, while unsaturated flow devices are at UKY, as are rainfall simulation devices to study erosion and runoff. Other outstanding facilities are available through CEINT and several TĪNĒ consortium members routinely access state-of-the-science
synchrotron x-ray facilities
in the U.S.: Advanced Photon Source (McNear), Advanced Light Source (McNear), National Synchrotron Light Source (Unrine, Bertsch), Stanford Synchrotron Radiation Lightsource (Lowry) and the Diamond Light Source (Neal) in the UK.


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