University of Kentucky

UK College of Agriculture

UK Plant Pathology

Peter Nagy's Laboratory
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1:   Wang, R. Y., and Nagy, P. D. 2008. Tomato bushy stunt virus Co-Opts the RNA-Binding Function of a Host Metabolic Enzyme for Viral Genomic RNA Synthesis. Cell Host Microbe 3:178-87.

2:   Pogany, J., Stork, J., Li, Z., and Nagy, P. D. 2008. In vitro assembly of the Tomato bushy stunt virus replicase requires the host Heat shock protein 70. Proc Natl Acad Sci U S A, 105: 19956-61.

3:   Li, Z., D. Barajas, T. Panavas, D. A. Herbst, and P. D. Nagy. 2008. Cdc34p ubiquitin-conjugating enzyme is a component of the tombusvirus replicase complex and ubiquitinates p33 replication protein. J Virol. 82: 6911-6926.

4:   Pogany, J., and Nagy, P. D. 2008. Authentic replication and recombination of Tomato bushy stunt virus RNA in a cell-free extract from yeast. J Virol 82:5967-80.

5:   Nagy, P. D. 2008. Yeast as a Model Host to Explore Plant Virus-Host Interactions. Annu Rev Phytopathol. 46:217-42.

6:   Rajendran, K. S., and Nagy, P. D. 2008. Surface plasmon resonance analysis of interactions between replicase proteins of tomato bushy stunt virus. Methods Mol Biol 451:267-77.

7:   Panavas, T., E. Serviene, J. Pogany, and Nagy, P. D. 2008. Genome-wide screens for identification of host factors in viral replication. Methods Mol Biol 451:615-24.

8:   Nagy, P. D., and J. Pogany. 2008. Multiple roles of viral replication proteins in plant RNA virus replication. Methods Mol Biol 451:55-68.

9:   Pathak, K. B., Sasvari, Z., and Nagy, P. D. (2008). The host Pex19p plays a role in peroxisomal localization of tombusvirus replication proteins. Virology 379: 294-305.

10:   Jonczyk, M., Pathak, K. B., Sharma, M., and Nagy, P. D. 2007. Exploiting alternative subcellular location for replication: Tombusvirus replication switches to the endoplasmic reticulum in the absence of peroxisomes. Virology 362(2), 320-30.

11:   Jaag, H. M., Stork, J., and Nagy, P. D. 2007. Host transcription factor Rpb11p affects tombusvirus replication and recombination via regulating the accumulation of viral replication proteins. Virology 368, 388-404.

12:   Cheng, C. P., Jaag, H. M., Jonczyk, M., Serviene, E., and Nagy, P. D. 2007. Expression of the Arabidopsis Xrn4p 5'-3' exoribonuclease facilitates degradation of tombusvirus RNA and promotes rapid emergence of viral variants in plants. Virology 368, 338-248.

13:  Serva, S. and Nagy, P. D. 2006. Proteomics analysis of the Tombusvirus replicase: Hsp70 molecular chaperone is associated with the replicase and enhances viral RNA replication Journal of Virology 80: 2162-2169.

14:  Jiang, Y. Elena Serviene, Jozsef Gal, Tadas Panavas and Nagy, P. D. 2006. Identification of essential host factors affecting tombusvirus RNA replication based on the yeast yTHC collection. Journal of Virology 80(15), 7394-7404.

15:   Cheng, C-P., Elena Serviene, and Nagy, P. D. 2006. Suppression of viral RNA recombination by a host exoribonuclease. Journal of Virology 80: 2631-2640.

16:   Serviene, E., Jiang, Y., Cheng, C. P., Baker, J., and Nagy, P. D. 2006. Screening of the Yeast yTHC Collection Identifies Essential Host Factors Affecting Tombusvirus RNA Recombination. Journal of Virology 80(3), 1231-41.

17:   Rajendran, K. S., and Nagy, P. D. 2006. Kinetics and functional studies on interaction between the replicase proteins of Tomato Bushy Stunt Virus: Requirement of p33:p92 interaction for replicase assembly. Virology 345(1), 270-9.

18:   Panavas, T., Stork, J., Nagy, P. D. 2006. Use of double-stranded RNA templates by the tombusvirus replicase in vitro: Implications for the mechanism of plus-strand initiation. Virology 352(1), 110-20.

19:   Nagy, P. D., and Pogany, J. 2006. Yeast as a model host to dissect functions of viral and host factors in tombusvirus replication. Virology 344(1), 211-20.

20:  Panavas, P. Elena Serviene, Jeremy Brasher, and P. D Nagy. 2005. Yeast genome-wide screen reveals dissimilar set of host genes affecting replication of RNA viruses. Proc Natl Acad Sci U S A. (PNAS) 102:7326-31.

21:   Stork, J., Z. Panaviene, and P. D. Nagy. 2005. Inhibition of in vitro RNA binding and replicase activity by phosphorylation of the p33 replication protein of Cucumber necrosis tombusvirus. Virology 343:79-92.

22:   Serviene, E., N. Shapka, C. P. Cheng, T. Panavas, B. Phuangrat, J. Baker, and Nagy, P.D. 2005. Genome-wide screen identifies host genes affecting viral RNA recombination. Proc Natl Acad Sci U S A. (PNAS) 102:10545-10550.

23:   Shapka, N., J. Stork, and P. D. Nagy. 2005. Phosphorylation of the p33 replication protein of Cucumber necrosis tombusvirus adjacent to the RNA binding site affects viral RNA replication. Virology 343:65-78.

24:   Cheng, C. P., T. Panavas, G. Luo, and P. D. Nagy. 2005. Heterologous RNA replication enhancer stimulates in vitro RNA synthesis and template-switching by the carmovirus, but not by the tombusvirus, RNA-dependent RNA polymerase: Implication for modular evolution of RNA viruses. Virology 341:107-21.

25:   Panaviene, Z., Panavas, T., and P. D Nagy. 2005. Role of an internal and two 3'-terminal RNA elements in assembly of tombusvirus replicase. Journal of Virology 79: 10608-18.

26:   Panavas, T., C. M. Hawkins, Z. Panaviene, and P. D. Nagy. 2005. The role of the p33:p33/p92 interaction domain in RNA replication and intracellular localization of p33 and p92 proteins of Cucumber necrosis tombusvirus. Virology. 338: 81-95.

27:   Tadas Panavas and Peter D. Nagy. 2005. Mechanism of stimulation of plus-strand synthesis by an RNA replication enhancer in a tombusvirus” Journal of Virology 79:9777-85.

28:   Pogany, J., White, K.A., and Nagy, P.D. 2005. Specific Binding of the Tombusvirus Replication Protein p33 to an Internal Replication Element in the Viral RNA is Essential for Replication. Journal of Virology 79:4859-69.

29:   Monkewich, S., Lin, H-X, Fabian, M.R., Xu, W., Na, H., Ray, D., Chernysheva, O.A., Nagy, P.D., and White, K.A. 2005. p92 Polymerase Coding Region Contains an Internal RNA Element Required at an Early Step in Tombusvirus Genome Replication. Journal of Virology, 79:4848-58.

30:   Panaviene, Z. Panavas, T., Serva, S. and Nagy, P.D. 2004. Purification of the Cucumber necrosis virus replicase from yeast: The role of co-expressed viral RNA in stimulation of replicase activity. Journal of Virology 78, 8254-8263.

31:   Rajendran, K. S. and Nagy, P.D. 2004. Interaction between the replicase proteins of Tomato Bushy Stunt Virus in vitro and in vivo. Virology, 326: 250-261.

32:   Boonrod, K., Galetzka, D., Nagy, P.D., Conrad, U. and Krczal, G. 2004. Single-chain antibodies against a plant viral RNA-dependent RNA polymerase confer virus resistance. Nature Biotechnolology, 22, 856-862.

33:   Panavas, T., and Nagy, P. D. 2003. Yeast as a model host to study replication and recombination of defective interfering RNA of Tomato bushy stunt virus. Virology 314: 315-325.

34:   Pogany, J., Fabian, M., White, K.A., and Nagy, P. D. 2003. A replication silencer element in an RNA virus. EMBO Journal 22: 5602-5611.

35:   Cheng, C.-P. and Nagy, P. D. 2003. Mechanism of RNA recombination in Carmo- and Tombusviruses: Evidence for Template Switching by the RNA-dependent RNA polymerase in vitro. Journal of Virology 77: 12033-12047.

36:   Panaviene, Z. and Nagy, P. D. 2003. Mutations in the RNA-binding domains of tombusvirus replicase proteins affect RNA recombination in vivo. Virology 317, 359-372.

37:   Shapka, N. and Nagy, P.D. 2004. The AU-rich RNA recombination hot spot sequence of Brome mosaic virus is functional in tombusviruses: Implications for the mechanism of RNA recombination. Journal of Virology 78, 2288-2300.

38:   White, K.A., and Nagy, P.D. 2004. Advances in the Molecular Biology of Tombusviruses: Gene Expression, Genome Replication and Recombination. Progress in nucleic acid research and molecular biology 78, 187-226.

39:   Rajendran, K.S. and Nagy, P.D. 2003. Characterization of the RNA-binding domains in the replicase proteins of tomato bushy stunt virus. Journal of Virology 77, 9244-9258.

40:   Panavas, T., and Nagy, P. D. 2003. The RNA replication enhancer element of tombusviruses contains two interchangeable hairpins that are functional during plus-strand synthesis. Journal of Virology, 77, 258-269.

41:   Panaviene, Z., Baker, J. M., and Nagy, P. D. 2003. The overlapping RNA-binding domains of p33 and p92 replicase proteins are essential for tombusvirus replication. Virology, 308, 191-205.

42:   Panavas, T., Panaviene, Z., Pogany J., and Nagy, P. D. 2003. Generation of a novel cis-acting replication element by promoter duplication in tombusviruses. Virology, 310, 118-129.

43:   Cheng, C.-P., Pogany, J., and Nagy, P. D. 2002. Mechanism of DI RNA formation in Tombusviruses: Dissecting the requirement for primer extension by the Tombusvirus RNA-dependent RNA polymerase in vitro. Virology, 304, 460-473.

44:   Panavas, T., Pogany, J., and Nagy, P. D. 2002. Analysis of minimal promoter sequences for plus-strand synthesis by the Cucumber necrosis virus RNA-dependent RNA polymerase. Virology, 296, 263-274.

45:   Panavas, T., Pogany, J., and Nagy, P. D. 2002. Internal initiation by the Cucumber necrosis virus RNA-dependent RNA polymerase is facilitated by promoter-like sequences. Virology, 296, 275-287.

46:   Rajendran, K. S., Pogany, J., and Nagy, P. D. 2002. Comparison of Turnip crinkle virus RNA-dependent RNA polymerase preparations expressed in E. coli or derived from infected plants. Journal of Virology, 76, 1707-1717.

47:   Nagy, P. D., Pogany, J., and Simon, A. E. 2001. In vivo and in vitro characterization of an RNA replication enhancer in a satellite RNA associated with Turnip crinkle virus. Virology, 288, 315-324.

48:   Nagy, P. D. and Pogany, J. 2000. Partial purification and characterization of Cucumber necrosis virus and Tomato bushy stunt virus RNA-dependent RNA polymerases: Similarities and differences in template usage between tombusvirus and carmovirus RNA-dependent RNA polymerases. Virology, 276: 279-288.

49:   Yoshinari, S., Nagy, P.D., Simon, A.E., and T.W. Dreher (2000). CCA initiation boxes without unique promoter elements support in vitro transcription by three viral RNA-dependent RNA polymerases. RNA: 6, 698-707.

50:   Nagy, P. D., Pogany, J., and Simon, A. E. (1999). RNA elements required for RNA recombination function as replication enhancers in vitro and in vivo in a plus-strand RNA virus. EMBO Journal 18: 5653-5665.

51:   Nagy, P. D., Ogiela, C., and Bujarski, J.J. (1999). Mapping sequences active in homologous recombination in brome mosaic virus: Prediction of recombination hot spots. Virology, 254: 92-104.

52:   Nagy, P. D., Zhang, C. and Simon, A.E. (1998). Dissecting RNA recombination in vitro: role of RNA sequences and the viral replicase. EMBO Journal, 17: 2392-2403.

53:   Nagy, P. D., and Simon, A. E. (1998a). In vitro characterization of late steps of RNA recombination in turnip crinkle virus I. Role of the motif1-hairpin structure. Virology, 249: 379-392.

54:   Nagy, P. D., and Simon, A. E. (1998b). In vitro characterization of late steps of RNA recombination in turnip crinkle virus II. The role of the priming stem and flanking sequences. Virology, 249: 393-405.

55:   Figlerowicz, M., Nagy, P. D., Tang, N., Kao, C.C., and Bujarski, J.J. (1998). Mutations in the N terminus of the brome mosaic virus polymerase affect genetic RNA-RNA recombination. Journal of Virology, 72: 9192-9200.

56:   Nagy, P. D., and Bujarski, J.J. (1998). Silencing homologous recombination hotspots with GC-rich sequences in brome mosaic virus. Journal of Virology, 72: 1122-1130.

57:   Nagy, P. D., and Simon, A.E. (1997). New insights into the mechanisms of RNA recombination. Virology, 235: 1-9.

58:   Nagy, P. D., Carpenter, C.D. and Simon, A.E. (1997). A novel 3’-end repair mechanism in an RNA virus. Proceedings of the National Academy of Sciences USA (PNAS), 94: 1113-1118.

59:   Figlerowicz, M., Nagy, P. D., and Bujarski, J.J. (1997). A mutation in the putative RNA polymerase gene inhibits nonhomologous, but not homologous, genetic recombination in an RNA virus. Proceedings of the National Academy of Sciences USA (PNAS) 94:2073-2078.

60:   Nagy, P. D., and Bujarski, J.J. (1997). Engineering of homologous recombination hotspots with AU-sequences in brome mosaic virus. Journal of Virology, 71: 3799-3810.

61:   Nagy, P. D., and Bujarski, J.J. (1996). Homologous recombination in brome mosaic virus: AU-sequences decrease the precision of crossovers. Journal of Virology, 70:415-426.

62:   Simon, A.E., and Nagy, P. D. (1996). RNA recombination in turnip crinkle virus: its role in formation of chimeric RNAs, multimers, and in 3’-end repair. Seminars in Virology 7: 373-379.

63:   Bujarski, J.J. and Nagy, P. D. (1996). Different mechanisms of homologous and nonhomologous recombination in brome mosaic virus: role of RNA sequences and replicase proteins. Seminars in Virology 7:363-372.

64:   Nagy, P. D., Dzianott, A., Ahlquist, P. and Bujarski, J.J. (1995). Mutations in the helicase-like domain of protein 1a alter the sites of RNA-RNA recombination in brome mosaic virus. Journal of Virology, 69: 2547-2556

65:   Nagy, P. D., and Bujarski, J.J. (1995). Efficient system of homologous RNA recombination in brome mosaic virus: sequence and structure requirements and accuracy of crossovers. Journal of Virology, 69: 131-140.

66:   Bujarski, J.J, Nagy, P. D., and Flasinski, S. (1994). Molecular studies of genetic RNA-RNA recombination in brome mosaic virus. Advances in Virus Research, Vol 43 (eds. Maramorosch, K., Murphy, F. and Shatkin, A). pp. 275-302.

67:   Pogany, J., Huang, Q., Romero, J., Nagy, P. D., Bujarski, J. J. 1994. Infectious transcripts from PCR-amplified broad bean mottle bromovirus cDNA clones and variable nature of leader regions in RNA. J Gen Virol. 75; 693-697.

68:   Bujarski, J.J, and Nagy, P. D. (1994). Targeting of the site of nonhomologous recombination in brome mosaic virus. Archives in Virolology 9: 231-238.

69:   Nagy, P. D., and Bujarski, J.J. (1993). Targeting the site of RNA-RNA recombination in brome mosaic virus with antisense sequences. Proceedings of the National Academy of Sciences USA (PNAS) 90: 6390-6394.

70:   Nagy, P. D., and Bujarski, J.J. (1992). Genetic recombination in brome mosaic virus: Effect of sequence and replication of RNA on accumulation of recombinants. Journal of Virology, 66: 6824-6828.

71:   Burgyan, J., Nagy, P. D., and Russo, M. (1990). Synthesis of infectious RNA from full-length cloned cDNA to RNA of cymbidium ringspot tombusvirus. Journal of General Virology, 71: 1857-1860.

72:   A. Ádám and Nagy P. D. (1989). Variations in membrane polar lipids of barley leaves infected with three strains of barley stripe mosaic virus and with poa semilatent virus. Plant Science, 61: 53-59.

73:   Nagy, P. D., Gaborjanyi, R., Kovacs, L., Farkas, I. 1989. Antiviral activity of tiazofurine against barley stripe mosaic virus. Antiviral Res. 11: 41-45.


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