University of Kentucky College of Agriculture

The Vaillancourt Laboratory

Department of Plant Pathology

University of Kentucky

Fungal Genetics and Plant Pathology Research

Laboratory Protocols

and Biological Collections

This page is still under construction!

Common Laboratory Protocols

Fungal DNA Prep

Everyday Midi-prep
QUICK Mini-prep
High-throughput DNA prep method

Fungal RNA Prep

Qiagen Method
Trizol Method
Northern Blot

DNA Quantification on the Fluorometer (pdf)

Use of the Lyophilizer (Freeze Dryer)

Bacterial colony PCR

Southern Blot

Protocol 1: Non-radioactive
Protocol II: Radioactive

Bacterial Electroporation (pdf)

Storage of Fungal Cultures

Staining Protocols

DAPI Nuclear Staining
Tissue Clearing
Viability Staining
Acridine Orange

Fungal Protoplast Prep

From Mycelium (pdf)
From Oval Spores (pdf)
From Oval Spores (Thon)

Fungal Transformation

Colletotrichum Quicker Method
Colletotrichum Slower Method (pdf)
Gibberella zeae
Agrobacterium-Mediated Transformation of Colletotrichum (pdf)

Fungal Matings

Glomerella graminicola
Gibberella zeae

Plant Growth and Inoculations

Growing Corn in the Greenhouse (pdf)
Using the Dew Chamber (pdf)
Corn Inoculations

Stalk Inoculations (pdf)
Leaf Inoculations
Serenella Sukno’s Leaf Anthracnose Assay

Media Recipes (pdf)

Biological Collections

Genomic and cDNA Libraries

Fungal Strains

Colletotrichum spp.
Fusarium graminearum
Schizophyllum commune
Diplodia sp.

Bacterial Strains

Plasmid Vectors


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