Sydowia 53: 44-73

Hybrid fungal endophytes symbiotic with the grass Lolium pratense.

Craven, K.D., J.D. Blankenship, A. Leuchtmann, K. Hignight, and C.L. Schardl. 2001.

Hybrid fungal endophytes symbiotic with the grass Lolium pratense. Sydowia 53:44-73. Classification of endophytes inhabiting Lolium pratense (= Festuca pratensis) based on morphological and molecular phylogenetic characteristics is reported. From an extensive survey of L. pratense collected throughout Europe, we concluded that the overwhelmingly predominant endophyte was Neotyphodium x uncinatum. We constructed phylogenies based on three unlinked, nuclear genes and analyzed allozymes to evaluate the relationship of N. x uncinatum to Epichlo?/i> endophytes. Our results suggest that N. x uncinatum is an interspecific hybrid of two Epichlo?/i> species. A second, much rarer hybrid endophyte was isolated from L. pratense which may have shared one of its two Epichlo?/i> ancestors with N. x uncinatum. This second isolate is a previously undescribed species for which we propose the name Neotyphodium x siegelii.