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CURRICULUM VITAE

R. Frank Cook, Ph.D.
rfcook1@pop.uky.edu
(859) 218-1119

Research Associate Professor
Department of Veterinary Science
M.H. Gluck Equine Research Center
College of Agriculture, Food & Environment
University of Kentucky

Professional Experience

2011 – Present: Research Associate Professor
Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky.

1998 – Present: Associate Member, Graduate Faculty
University of Kentucky.

1998 – 2011: Research Assistant Professor
Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky.

1991 - 1998: Research Specialist
Department of Veterinary Science, Gluck Equine Research Center, University of Kentucky, Lexington, Kentucky.

1989 - 1991: NIH Postdoctoral Researcher
Laboratories of Molecular and Development Biology. Ohio Agricultural Research and Development Center. The Ohio State University, Department of Dairy Science 1680, Madison Avenue, Wooster, Ohio 44691.

1983 - 1988: Virologist/Project Leader
Equine Virology Unit, Animal Health Trust, Lanwades Park, Kennett,
Newmarket, Suffolk, Great Britain.

1979 - 1983: Postdoctoral Research Fellow: Tumour Virus Research Group.
University of Warwick, Department of Biological Sciences, Coventry, CV4 7AL Great Britain.

Extramural Research Funding:

The development of an ISCOM vaccine against EHV-1. Principal Investigator: J .A. Mumford. Co- Principal Investigators: R. F. Cook and D. Hannant. Agency:  The Horserace Betting Levy Board. Period:  1985-1988. Amount:  £190,000

Molecular analysis of antigenic and genetic variation in equid herpes virus type 1 (subtype 1 and 2) isolates in the United Kingdom. Principal Investigator: R. F. Cook. Agency:  Equine Virology Research Foundation. Period:  1986-1988. Amount:  £60,700

Regulation of EIAV Replication and Disease. Principal Investigators: R. C. Montelaro, C. J. Issel. Co-Investigator:  R. F. Cook. Agency:  NIH/NIAID Type R01 CA49296. Period:  1996-2001. Amount:  $1,592,313

EIA Vaccine Trial. Principal Investigators: R. C. Montelaro, C. J. Issel. Co-Investigator:  R. F. Cook. Agency:  Intervet Corp. Period:  2000-2002. Amount:  $316,000

Prospective study of progeny from inapparent carriers of EIA in a herd of wild Indian horses in Southwest Oklahoma. Principal Investigator: C. J. Issel. Co-Investigator:  R. F. Cook. Agency:  College of Veterinary Medicine, Oklahoma State University. Period:  1997-2003. Amount:  $5000 

EIAV Envelope Variation and Vaccine Efficacy. Principal Investigator: R. C. Montelaro
Co-Investigator: R. F. Cook. Agency:  NIH R01 AI25850. Period:  2003 - 2008. Amount:  $1,091,261.

Equine Infectious Anemia Diagnostics. Principal Investigator: C. J. Issel. Co-Investigator: R. F. Cook. Agency:  Idexx Laboratories. Period:  2006-2011. Amount:  $409,000.

Development of Gene Delivery Approaches to Treat Equine Respiratory Diseases. Principal Investigator: D. W. Horohov. Co-Principal Investigator: R. F. Cook. Agency:  Kentucky Science & Engineering Foundation. Period:  2006. Amount:  $49,573.

EIAV Envelope Variation and Vaccine Efficacy. Principal Investigator: R. C. Montelaro. Co-Principal Investigator: R. F. Cook. Agency:  NIAID/NIH R01A1025850-21A2. Period:  009-2014. Amount:  UK 5 year budget proposed at $1,737,500.

Molecular characterization of neurovirulent EHV-1 strains. Principal Investigator: U. B. R. Balasuriya. Co-Principal Investigator: R. F. Cook. Agency:  Grayson-Jockey Club Research Foundation, Inc. Period:  2010-2012. Amount:  $95,361.

Comparison of in vitro antiviral activity of herpesvirus DNA polymerase inhibitors against neuropathogenic and non-neuropathogenic strains of equine herpesvirus-1. Principal Investigator: U. B. R. Balasuriya. Co-Principal Investigator: R. F. Cook. Agency:  Kentucky Horse Racing Commission. Period:  2010-2011. Amount:  $40,499.

Testing the susceptibility of neuropathogenic and non-neuropathogenic equine herpesviruses-1 strains to antivirals. Principal Investigator: U. B. R. Balasuriya. Co-Principal Investigator: R. F. Cook. Agency:  American Quarter Horse Foundation. Period:  2010-2011.
Amount:  $39,745.

Development of a genetically defined live attenuated equine herpesvirus-1 vaccine. Principal Investigator: U. B. R. Balasuriya. Co-Principal Investigator: R. F. Cook. Agency: Elanco Animal Health. Period: 2012-2014. Amount: $163,752.

Identification of genetic factors responsible for establishment of equine arteritis virus carrier state in stallions. Principal Investigator: U. B. R. Balasuriya. Co-Principal Investigator: R. F. Cook. Agency: USDA-AFRI. Period: 2013–2017.  Amount: $2,925,456.

Development of Recombinant Vaccines against Porcine Reproductive and Respiratory and Syndrome (PRRS): Equine Arteritis Virus-Vectored Chimeric Vaccines against PRRS. Principal Investigator:  U. B. R. Balasuriya. Co-Principal Investigator:  R. F. Cook. Agency: Merial. Period: 2013-2015. Amount: $444,571.

Intramural Research Funding:

Generation of infectious cDNA clones from biologically significant mutants of an equine lentivirus. Principal Investigator: C. J. Issel. Co-Principal Investigator: R. F. Cook. Agency:  Research Committee Grant, University of Kentucky. Period:  1992-1993. Amount:  $10,500.

Host Factors Involved in Equine Infectious Anemia. Principal Investigator: R. F. Cook. Agency:  Research Committee Grant, University of Kentucky. Period:  1999-2000. Amount:  $3,500.

Concepts in HIV Vaccine Research: Enhanced Responses to Genetic Immunization with Lentiviruses. Principal Investigator: R. F. Cook. Agency:  Research Support Grant, University of Kentucky. Period:  December 1, 2005 to November 30, 2006. Amount:  $16,282.

Comparative Genomics of Gamma-Herpesviruses of Equids. Principal Investigator: U. B. R. Balasuriya. Co-Principal Investigator: R. F. Cook. Agency:  Paul Mellon Post-Doctoral Scholarship Fund. Period:  2009-2011. Amount:  Postdoctoral Stipend for Two Years.

University of Kentucky, 2008-2009 Research Activity Awards (Purchase of peptides to investigate immune responses to DNA vaccines). Principal Investigator: R. F. Cook. Agency:  University of Kentucky. Amount:  $1,318.

University of Kentucky, 2008-2009 Research Activity Awards (Development of a universal qRT-PCR for EIAV). Principal Investigator: R. F. Cook. Agency:  University of Kentucky. Amount:  $1,200.

University of Kentucky, 2009-2010 Research Activity Awards (Development of an adenoviral vector expressing equine IL-15). Principal Investigator: R. F. Cook. Agency:  University of Kentucky. Amount:  $1,648.

Molecular Epidemiology and Improved Diagnostics for Equine Infectious Anemia Virus (EIAV). Principal Investigator: R. F. Cook. Agency:  Gluck Equine Research Center. Period:  2009-2010. Amount:  $5,000.

Improved DNA Vaccines: Evaluating Cell Mediated Immune Responses. Principal Investigator: R. F. Cook. Agency:  Gluck Equine Research Center ($8,530). Period:  2009-2010. Amount:  $7,500.

Infectivity and Pathogenicity of Full Length Molecular Clones Derived From Equine Infectious Anemia Virus (EIAV) Strains Isolated in Ireland During 2006. Principal Investigator: R. F. Cook. Agency: Clay Fellowship Fund, Gluck Equine Research Center. To support Dr Michelle Quinlivan, Irish Equine Centre. Period: 2011. Amount: $15,355.

Other Funding:

Pathogenesis of Equine Infectious Anemia. Principal Investigators: C. J. Issel, R. F. Cook. Agency:  *CRIS/Kentucky Agricultural Experiment Station. Period:  2001-2006.

Control of Equine Infectious Anemia. Principal Investigators: C. J. Issel, R. F. Cook Agency:  *CRIS/Kentucky Agricultural Experiment Station. Period:  2006-2011. *Federal funding provided to State Agricultural Experiment Stations under Hatch Act of 1887. In 2010, the University of Kentucky received $1,035,400.

Selected peer-reviewed publications (in chronological order)

Detection of influenza nucleoprotein antigen in nasal secretions from horses infected with equine influenza (H3N8) viruses. R. F. Cook, R. Sinclair and J. A. Mumford. (1988). J. Virol. Methods 20, 1 - 12.

The characterization of neutralizing and non-neutralizing monoclonal antibodies against equine herpes virus type 1. R. Sinclair, R. F. Cook and J. A. Mumford (1989). J. of General Virology 70, 455 - 459.

Protection against lethal equine herpesvirus type 1 (subtype 1) infection in hamsters by immune stimulating complexes containing the major viral glycoproteins. R. F. Cook, T. O'Neill, R. Sinclair, B. Sundqvist and J. A. Mumford. (1990). Vaccine 8, 491 - 496.

Retrotransposon gene engineering. R. F. Cook, S. J. Cook and C. P. Hodgson. (1991). Nature Biotechnology 9, 748 - 751.

Liver-specific expression of a phosphenopyruvate carboxykinase-neo gene in genetically modified chickens. (1993). R. F. Cook, S. J. Cook, S. Savon, M. McGrane, M. Hartitz, R. W. Hanson and C. P. Hodgson. Poultry Science 72 : 554 - 567.

Enhanced sensitivity to neutralizing antibodies in a variant of Equine Infectious Anemia Virus (EIAV) is linked to amino acid substitutions in the surface unit (SU) envelope glycoprotein. (1995). R. F. Cook, S. L. Berger, K. E. Rushlow, J. J. McManus, S. J. Cook, S. Harrold, M. L. Raabe, R. C. Montelaro and C. J. Issel. J. Virol. 69 : 1493 - 1499.

Replication in vitro and in vivo of an equine infectious anemia virus (EIAV) mutant deficient in deoxyuridine triphosphate (dUTPase) activity. (1995). D. L. Lichtenstein, K. E. Rushlow, R. F. Cook, M. L. Raabe, C. J. Swardson, G. J. Kociba, C. J. Issel, and R. C. Montelaro. J. Virol. 69 : 2881 - 2888.

Development and characterization of an in vivo pathogenic molecular clone of equine infectious anemia virus. (1998). R. F. Cook, C. Leroux, S. J. Cook, S. L. Berger, D. L. Lichtenstein, N. N. Ghabrial, R. C. Montelaro and C. J. Issel. J. Virol. 72 (2) : 1383-1393.

Immunization with a recombinant envelope protein (rgp90) of EIAV produces a spectrum of vaccine efficacy ranging from lack of clinical disease to severe enhancement. (1998) M. Raabe, C. J. Issel, S. J. Cook, R. F. Cook, B. Woodson and R. C. Montelaro. Virology 245 : 151-162.

Effects of long terminal repeat sequence variation on equine infectious anemia virus replication in vitro and in vivo. (1999). D. L. Lichtenstein, J. K. Craigo, C. Leroux, K. E. Rushlow, R. F. Cook, S. J. Cook, C. J. Issel and R. C. Montelaro. Virology 263 : 408-417

Tissue sites of persistent and active replication of equine infectious anemia virus during acute and asymptomatic infection in experimentally infected equids.(2000) S. M. Harrold, S. J Cook, R. F. Cook, K. E. Rushlow, C. J. Issel and R. C. Montelaro. J. Virol 74 (7) : 3112-3121

Differential responses of Equus caballus and Equus asinus to infection with two pathogenic strains of equine infectious anemia virus. S.J. Cook, R.F. Cook, R.C. Montelaro and C.J. Issel (2001). Veterinary Microbiology, 79, (2):93-109

Multiple RNA splicing and the presence of cryptic RNA splice donor and acceptor sites may contribute to low expression levels and poor immunogenicity of potential DNA vaccines containing the env gene of equine infectious anemia virus (EIAV). W. Zhou, R. F. Cook, S. J. Cook, S. A. Hammond, K. Rushlow, N. N. Ghabrial, S. L. Berger, R. C. Montelaro, and C. J. Issel, (2002). Veterinary Microbiology, 88 : 127-151.

Development of a multiplex real-time reverse transcriptase-polymerase chain reaction for equine infectious anemia virus (EIAV). R. F. Cook, S. J. Cook, F. Li, R. C. Montelaro and C. J. Issel, (2002). Journal of Virological Methods, 105 : 171-179.

Enhancement of equine infectious anemia virus (EIAV) virulence by identification and removal of sub-optimal nucleotides. (2003). R. F. Cook, S. J. Cook, S. l. Berger, C. Leroux, N. N. Ghabrial, M. Gantz, P. S. Bolin, M. R. Mousel, R. C. Montelaro and C. J. Issel. Virology, 313 : 588-603.

Genetic immunization with codon-optimized equine infectious anemia virus (EIAV) surface unit (SU) envelope sequences stimulates immune responses in ponies. (2005). R. F Cook, S. J. Cook, P. S. Bolin, L. J. Howe, R. C. Montelaro and C. J. Issel. Veterinary Microbiology, 108: 23-37.

Evidence of an oscillating peripheral clock in an equine fibroblast cell line and adipose tissue, but not in peripheral blood. (2006) B. A. Murphy, M. M. Vick, D. R. Session, R. F. Cook, B. P. Fitzgerald. Journal of Comparative Physiology A. 192: 743-751.
 
Acute inflammation transiently synchronizes clock gene expression in equine peripheral blood. (2007). B. A. Murphy, M. M. Vick, D. R. Sessions, R. F. Cook and B. P. Fizgerald.  Brain Behavior Immunity. 21: 467-476.
 
Relationship between inflammatory cytokines, obesity and insulin sensitivity in the horse. (2007). M.M. Vick, A.A. Adams, B.A. Murphy, D.R. Sessions, D.W. Horohov, R.F. Cook, B.J. Shelton and B.P. Fitzgerald.  Journal of Animal Science, 85:1144-1155.
 
Real-time quantitative RT-PCR and PCR assays for a novel European field isolate of equine infectious anaemia virus based on sequence determination of the gag gene. (2007). M. Quinlivan, R.F. Cook, and A. Cullinane. Veterinary Record 160:611-618.

Systemic inflammation influences insulin resistance in the horse and increases inflammatory cytokine expression in equine adipose tissue. (2008).  M.M. Vick, B.A. Murphy, D.R. Sessions, S.E. Reedy, E.L. Kennedy, D.W. Horohov, R.F. Cook, and B.P. Fitzgerald.  Amer. J.of Vet. Res. 69:130-139.

Equine neutrophils respond to PGE2 by activating expression of core circadian clock genes. (2008). B.R. Murphy, C. Merant, M.M. Vick, R.F. Cook, S.A. Brooks, D.W. Horohov and B.P. Fitzgerald. The Open Veterinary Science Journal. 2:96-103.

Full-length and internally deleted forms of interleukin-7 are present in horse (Equus caballus) lymph node tissue. (2008). R.F. Cook, S.J. Cook, D.L. Even, C. Schaffer and C.J. Issel. Veterinary Immunology and Immunopathology. 125:126-134.

Equine neutrophils respond to PGE2 by activating expression of core circadian clock genes. (2008). B. R. Murphy, C. Merant, M. M. Vick, R. F. Cook, S. A. Brooks, D. W. Horohov and B. P. Fitzgerald. The Open Veterinary Science Journal. 2: 96-103.

Equine infectious anaemia. (2009). R. F. Cook*, S. J. Cook and C. J. Issel. Equine Veterinary Education Manual: Infectious Diseases of the Horse Ed T. S. Mair and R. E. Hutchinson 56-71.

Approccio biomolecolare alla diagnosi di anemia infettiva equine. (2009). F. Passamonti, K. Cappelli, S. Capomaccio, M. Felicetti, M. L. Marenzoni, G. Coppola, A. Testa, R. F. Cook, A. Verini Supplizi and M. Coletti. Ippologia (1120-5776), 4, 20, 27-32

The increased prevalence of neuropathogenic strains of EHV-1 in equine abortions. (2010). K. L. Smith, G. P. Allen, A. J. Branscum, R. F. Cook, M. L. Vickers, P. J. Timoney, and U. B. R. Balasuriya. Veterinary Microbiology. 141: 5-11.

Complex Interactions between the Major and Minor Envelope Proteins of Equine Arteritis Virus Determine its Tropism for Equine CD3+ T Lymphocytes and CD14+ Monocytes. (2010). Y. Y. Go, J. Zhang, P. J. Timoney, R. F. Cook, D. W. Horohov, and U. B. R. Balasuriya. J. Virol. 84: 4898-4911.

Relationship between equine herpesvirus-1 myeloencephalopathy and viral genotype. (2010). S. Pronost,R. F. Cook, G. Fortier, P. J. Timoney and U. B. R. Balasuriya. Equine Veterinary Journal 42:672-674.

Molecular detection, epidemiology and genetic characterization of novel European field isolates of equine infectious anemia virus. (2011). K. Cappelli, S. Capomaccio, R. F. Cook, M. Felicetti, M. L. Marenzoni, G. Coppola, A. Verini-Supplizi, M. Coletti and F. Passamonti. J. Clin. Micro. 49:27-33.

Isolation and characterization of β-haemolytic-Streptococci from endometritis in mares. (2011). P. Casagrande Proietti, A. Bietta, G. Coppola, M. Felicetti, R. F. Cook, M. Coletti, M. L. Marenzoni and F. Passamonti. Veterinary Microbiology 152:126-130.

Assessment of correlation between in vitro CD3+ T cell susceptibility to EAV infection and clinical outcome following experimental infection. (2012) Y. Y. Go, R. F. Cook, J. Q. Fulgencio, J. R. Campos, P Henney, P. J. Timoney and U. B. R. Balasuriya. Veterinary Microbiology 157: 220-225.

Detection, Molecular Characterization and Phylogenetic Analysis of Full-Length Equine Infectious Anemia (EIAV) Gag Genes isolated from Shackleford Banks Wild Horses. (2012) S. Capomaccio, Z. A. Willand, S. J. Cook, C. J. Issel, E. M. Santos, J. K. P. Reis and R. F. Cook.* Veterinary Microbiology 157: 320-332.

Geographic structuring of global EIAV isolates: a single origin for New World strains? (2012). S. Capomaccio, K. Cappelli, R. F. Cook, F. Nardi, R. Gifford, M. L. Marenzoni, and F. Passamonti. Virus Research 163: 656-659.

A New Allelic Discrimination Real-Time PCR Assay for Detection and Differentiation of A2254 and G2254 Strains of EHV-1. (2012). K. L. Smith, Y. Li, P. Breheny, R. F. Cook, P. J. Henney, S. Sells, S. Pronost, Z. Lu, B. M. Crossley, P. J. Timoney, and U. B. R. Balasuriya. Journal of Clinical Microbiology 50: 3138-3147.

Development of a nested PCR assay to detect equine infectious anemia proviral DNA from peripheral blood of naturally infected horses. (2012) J.-B.  Dong, W. Zhua, R. F. Cook, Y. Goto, Y. Horii  and T. Haga. Archives of Virology (in press)

The determination of in vivo envelope-specific cell-mediated immune responses in equine infectious anemia virus-infected ponies. (2012). C. Liu, R. F. Cook, S. J. Cook, J. K. Craigo, D. L. Even, C. J. Issel, R. C. Montelaro and D. W. Horohov. Veterinary Immunology and Immunopathology (in press).

Challenges and proposed solutions for more accurate serological diagnosis of equine infectious anaemia. (2012) C. J. Issel, M. T. Scicluna, S. J. Cook, R. F. Cook, A. Caprioli, I. Ricci, F. Rosone, J. K. Craigo, R. C. Montelaro and G. L. Autorino.  Veterinary Record. (in press)

Identification of a novel equine infectious anemia virus (EIAV) field strain isolated from feral horses in Southern Japan. (2012). J-B. Dong, W. Zhu, R. F. Cook, Y. Goto, H. Horii and T. Haga. J. Gen. Virol. 2012 [Epub 2012 Oct 24].

Genetic characterisation by composite sequence analysis of a new pathogenic field strain of equine infectious anaemia virus (EIAV) from the 2006 outbreak in Ireland outbreak in Ireland. (2012). M. Quinlivan, F. Cook, R. Kenna, S. Callinan and A. Cullinane. J. Gen Virol  2012 Nov 21. [Epub ahead of print].

Selected Book Chapters and Articles

Equine Infectious Anemia Virus. R. F. Cook, C. J. Issel and R. C. Montelaro. (1996). Virus Infections of Equines. pp 295-323. Ed. by M. J. Studdert. Elsevier Science B.V.

Equine Infectious Anemia. R. F. Cook and C. J. Issel. (2002) 9th Edition of the McGraw-Hill Encyclopedia of Science and Technology Volume 6, pp 640-641.

Infectious diseases of livestock with special reference to Southern Africa. Second Edition. Equine infectious anaemia. R. F. Cook and C. J. Issel. (2005) Ed. By J. A. W. Coetzer and R. C. Tustin Volume 2, Chapter 62, pp 747-751. Oxford University Press-Southern Africa.

From petunias to a worm, to horses: the Nobel Prize in Medicine and siRNA. R. F. Cook and D. W. Horohov. Research Today Grayson-Jockey Club Foundation Newsletter (2006) Volume 23, No 4, p 1-4.

International Collaborative Studies

Ireland
Collaboration with Professor A. Cullinane and Dr M. Quinlivan, Virology Unit, Irish Equine Center, Johnstown, County Kildare, Ireland on the full length molecular cloning of viral strains isolated during the 2006 Irish EIA outbreak. 2006-present.

Italy
Collaboration with Professor Fabrizio Passamonti Dr Stefano Capomaccio and Dr Katia Cappelli, Section of Infectious Disease, Dept. of Veterinary Pathology, Diagnostics and Clinic Faculty of Veterinary Medicine, University of Perugia, Italy on the molecular characterization of EIAV strains isolated in Italy. 2008-present.

Italy
Collaboration with Dr G. Luca Autorino, Centro di Referenza Nationale per le Malattie degli Equini, Rome Italy on EIAV diagnostics and the study of EIAV infections in mules. 2008-present.

Brazil
Collaboration with Dr J.K.P. Reis, Universidade Federal de Minas Gerais, Brazil on the molecular characterization of South American EIAV Strains. 2009-present.

International Professional Service and Outreach

Ireland
At the request of the Irish Thoroughbred Breeders’ Association and Professor A. Cullinane, Head of Virology at the Irish Equine Center, advised and instructed (July 26 to August 2, 2006) Center and Irish Ministry of Agriculture personnel in the latest diagnostic techniques for EIA in response to the 2006 outbreak of this disease in Ireland.

United Kingdom
Provided consultation in 2008-2009 with Dr Graeme Cooke (Department for Environment, Food and Rural Affairs (DEFRA), International Animal Health, London who at the request of the European Union is designing a management plan to control EIAV in Romania where the virus is endemic (number of infected carrier horses estimated at 12,000).

Argentina
Following an outbreak of EIA on a Thoroughbred/Polo Pony farm in Argentina in 2009-2010 provided extensive advice on diagnosis, transmission and control of this disease to Drs Nicolas Schwint and Teótimo Becú, Médico Veterinario, Clínica Equina SRL, Buenos Aires, Argentina.  Drs Schwint Becú are responsible for educating Argentinean Veterinary Practitioners concerning the diagnosis of EIA.

Faculty Assignments:

Department of Veterinary Science Service

Standing Committee Membership: Animal Resources Advisory Committee (1998-2005)

Paul Mellon Postdoctoral Scholarship Advisory and Selection Committee (2000-2005)

Communications Advisory Committee (2005-2008)

Faculty Performance Advisory Committee (2005-2008)

Advisory & Strategic Planning Committee (2008-present)

Animal Resources Advisory Committee (2008-present)

Ad Hoc Faculty Committee: Janet H. Koller Bequest (2005)

Organizer for the Departmental Seminar Series 1999-2003 (Graduate course 770)

 

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