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Visiting Scientists at the Gluck Equine Research Center

Dr. K. Natarajaseenivasan

Medical Microbiology Laboratory
Department of Microbiology
School of Life Sciences
Bharathidasan University
Tiruchirappalli – 620 024
Tamilnadu, INDIA

Telephone: +91-431-2407082
Fax: +91-431-2407045

Photo of Dr. NatarajaseenivasanAs a visiting scientist of Gluck Equine Research Center, he is undergoing his long term ICMR (DHR) training program under the supervision of Prof. John F. Timoney, Keeneland Chair of Infectious Diseases.  

The duration of his visit is for a year from October 15, 2012 to October 14, 2013 and sponsored by Indian Council of Medical Research, Department of Health Research (DHR), Ministry of Health and Family Welfare, Government of India.

Background / Project

Dr. K. Natarajaseenivasan is a Professor and Principal Investigator in the Medical Microbiology Laboratory at the Department of Microbiology Bharathidasan University. He has received his post graduation in Microbiology from Madurai Kamaraj University, and was trained in Leptospirosis at Tamil Nadu Dr. MGR Medical University, where he has accomplished with Ph.D in Basic Medical Sciences. Previously he was a Research Officer at WHO Collaborative Centre for Diagnosis, Research, Reference and Training in Leptospirosis, Indian Council of Medical Research (ICMR), Port Blair, Andaman and Nicobar Islands.

He is interested to establish a continuous surveillance system and disease monitoring for medically important diseases like Leptospirosis, Salmonellosis, Ovarian and Cervical Carcinoma.  His current research provides new insights into microbial pathogenesis, novel protein and genetic markers for the early diagnosis of Leptospirosis.

His research trainings are focused in the following aspects.

1. Peptide based immuno pathogenesis for Leptospira interrogans serovar Autumnalis strain N2

2. Construction of Genomic DNA expression Library for Leptospira interrogans serovar Australis & Grippotyphosa

3. Molecular epidemiology for the transmission dynamics of L. interrogans  serovar Bratislavain Kentucky

Publications in Peer-reviewed Journals

  1. S Shanmughapriya, A Lency Francis, S Kavitha, K Natarajaseenivasan. In vitro actinomycetes biofilm development and biofilm inhibition by polyene antibiotic, nystatin on IUD copper surfaces. Biofouling 2012, 28: 929-935.  

  2. K Vinodhini, S Shanmughapriya, S Sanmugham, BC Das, K Natarajaseenivasan. Prevalence of High Risk Human Papillomavirus (HR-HPV) and its associated risk factors among Cervical Carcinoma Cases in Tiruchirappalli, Tamilnadu. International Journal of Gynecology and Obstetrics 2012, DOI 10.1016/j.ijgo.2012.06.019.

  3. Thilagavathi, S. Shanmughapriya, K. Vinodhini, K. Natarajaseenivasan and BC Das. Prevalence of Human Papillomavirus (HPV) among college going girls using self collected urine samples from Tiruchirappalli, Tamilnadu. Archieves of Gynecology and obstetrics 2012, DOI 10.1007/s00404-012-2500-6.

  4. K. Natarajaseenivasan, S. Shanmughapriya, R. Latha, G.B. Artiflavia, D. Senthil Pragash.  Prevalence of community acquired MRSA (CA-MRSA) in skin and soft tissue infections among cases from Puducherry, India.  J Public Health, 2012, DOI 10.1007/s10389-012-0507-x.

  5. K. Natarajaseenivasan, C. Lakshmi Priya, S. Vanithamani, S. Shanmughapriya, S.Anandhagiri.  Humoral immune response of inactivated bivalent Leptospira vaccine among dogs in Tiruchirappalli, Tamilnadu, India. World Journal of Vaccine, 2012, 2: 85-90.

  6. C. Lakshmipriya, S. Anandhagiri and K. Natarajaseenivasan. Diagnosis of canine leptospirosis by 16S rRNA nested PCR. Indian Journal of Animal Sciences, 2012, 82: 37-40.

  7. S. Shanmughapriya, G. Senthilkumar, K. Vinodhini, B C Das, Vasanthi Nachiappan, K. Natarajaseenivasan.  Viral and bacterial etiologies of epithelial ovarian cancer.  Eur J Clin Microbiol Infect Dis, 2012,  31:  2311-2317.

  8. K. Natarajaseenivasan, V. Raja and R. Narayanan. Rapid diagnosis of leptospirosis in patients with different clinical manifestations by 16s rRNA gene based Nested PCR. Saudi Journal of Biological Sciences, 2012, 19: 151-55.

  9. K. Vinodhini, S. Shanmughapriya, BC Das and K. Natarajaseenivasan.  Prevalence and risk factors of HPV infection among women from various provinces of the world.  Arch Gynecol Obstet, 2012, 284: 771-77.

  10. K. Natarajaseenivasan, S. Shanmughapriya, S. Velineni, S.C. Artiushin and J.F. Timoney.  Gene cloning, expression, and homology modeling of groEL of Leptospira interrogans serovar Autumnalis strain N2.  Genomics, Proteomics & Bioinformatics, 2011, 9: 151-157.

  11. A. Sankaranarayanan, Suresh SS Raja, K. Ponmurugan, S. Sarath Chandra Kandekar Subramanian, K. Natarajaseenivasan, A. Maripandi and Qaher A. Mandeel. Antibacterial activity of selected medicinal plants against Multiple Antibiotic Resistant Uropathogenes - A study from Kolli hills, Tamil Nadu, India.  Beneficial microbes, 2011, 2: 53-61.

  12. Shanmughapriya S, G. Senthil Kumar, K. Balakrishnan, Vasanthi Nachiappan, K. Vinodhini, K. Natarajaseenivasan.  Bilateral ovarian teratoma complicated with carcinosarcoma in a 68 year old woman: a case report.  BMC Cancer, 2011, 11:218.

  13. K. Natarajaseenivasan, Vedhagiri K, Sivabalan V, Prabagaran SG, Sukumar S, Artiushin SC and Timoney JF.  Seroprevalence of Leptospira borgpetersenii serovar Javanica infection among dairy cattle, rats and humans in the Cauvery river valley of southern India.  South East Asian J of Tropical Medicine and Hygiene, 2011, 42: 679.

  14. K. Natarajaseenivasan, S.C. Artiushin, S. Velineni, K. Vedhagiri, P. Vijayachari, J.F. Timoney.  Surface-associated Hsp60 chaperonin of Leptospira interrogans serovar Autumnalis N2 strain as an immunoreactive protein.  Eur J Clin Microbiol Infect Dis, 2011, 30:  1383-1389.

  15. T. Sathiamoorthi, S. Dhanapaul, K. Vedhagiri, K. Natarajaseenivasan.  Prevalence of multidrug resistant Salmonella enteric serotype typhi and paratyphi A of human cases from the Tiruchirappalli district, India and their associated virulent markers.  J Public Health, 2011, 19: 517-525.

  16. John F. Timoney,  K. Natarajaseenivasan, S. Velineni, J.M. Donahue, S.C. Artiushin, M. Fettinger.  A unique genotype of Leptospira interrogans serovar Pomona type kennewicki is associated with equine abortion.  Veterinary Microbiology, 2010, DOI 10.1016/j.vetmic.2011.02.049.

  17. G.B. Arti Flavia and K. Natarajaseenivasan.  Phylogenetic analysis of H1N1 sequences from pandemic infections during 2009 in India.  Bioinformation, 2011, 5: 416-421.

  18. Natarajaseenivasan K, Vijayachari P,  Sharma S,   Sugunan A.P,   Vedhagiri K,  Selvin J,  Sehgal S.C.  flaB PCR based identification for the determination of pathogenic leptospiral isolates.  Journal of Microbiology, Immunology and Infection, 2010,1:62-68.

  19. Jeyakumar N, Ranjitsingh AJA, Natarajaseenivasan K, Joseph I.   Molecular Characterisation Of Leptospiral Isolates Identified In Agricultural Labours Of Salem District Of Tamilnadu.  The Internet Journal of Microbiology™, 2010, ISSN: 1937-8289.

  20. Vedhagiri K, Natarajaseenivasan K,  Prabhakaran SG, Selvin J, Narayanan R, Shouche YS, Vijayachari P,  Ratnam S.  Characterization of Leptospira borgpetersenii isolates from field rats (Rattus norvegicus) by 16S rRNA and lipL32 gene sequencing.  Brazilian J Microbiology, 2009, 40:150-157.

  21. Vedhagiri K,  Manilal A,  Valliyammai T,  Shanmughapriya S,   Suganthan S,  Selvin J,    Natarajaseenivasan K.  Antimicrobial potential of a marine seaweed Asparagopsis taxiformis against Leptospira javanica isolates of rodent reservoirs.  Annals of Microbiology, 2009, 59: 1-7.

  22. Hema T.A, Gandhimathi R,  Seghal Kiran G, Joseph Selvin, Aseer Manilal,  Sujith S,  Natarajaseenivasan K. Optimization of biosurfactant production by sponge associated marine fungi Penicillium pfefferianum MSF5. Biotechnology and Bioprocess Engineering,2009 (In Press).

  23.  Vedhagiri K,  Natarajaseenivasan K,  Chellapandi P,  Prabhakaran S.G,  Selvin J, Sharma S,  Vijayachari P. Evolutionary implication of outer membrane lipoprotein encoded genes, ompL1, lipL32 and lipL41 of pathogenic Leptospira species.  Genomics, Proteomics & Bioinformatics, 2009, 7 (3):  96-106.

  24. Sugunan AP, Vijayachari P, Sharma S, Roy S, Manickam P, Natarajaseenivasan K, Gupte MD, Sehgal SC.  Risk factors associated with leptospirosis during an outbreak in Middle Andaman, India.  Indian J of Medical Research, 2009, 130 (1):  67-73.

  25. Joseph Selvin,  Shanmughapriya S,  Gandhimathi R,  Seghal Kiran G,  Rajeetha Raviji T,  Natarajaseenivasan K,  Hema T.A. Optimization and Production of Novel Antimicrobial Agents from Sponge Associated Marine Actinomycetes Nocardiopsis dassonvillei MAD08. Applied Microbiology and Biotechnology, 2009, 83 (3):  435-45.

  26. Kiran GS, Hema TA, Gandhimathi R, Selvin J, Thomas TA, Rajeetha RT, Natarajaseenivasan KOptimization and production of a biosurfactant from the sponge-associated marine fungus Aspergillus ustus MSF3.  Colloids Surf B Biointerfaces, 2009, 73(2):  250-6.

  27.  Kumar VG,  Latha R,  Vedhagiri K,  Sathiamoorthi T,  Jayarani G,  Sasikala R,  Selvin J,  Natarajaseenivasan K.  Phospholipase C, proteinase and hemolytic activities of Candida spp. isolated from pulmonary tuberculosis patients.   Journal of Medical Mycology, 2009, 19:  3-10.

  28. Natarajaseenivasan K,  Vijayachari P,  Sharma S, Sugunan AP,  Selvin J,  Sehgal SC.  Serodiagnosis of severe leptospirosis:  evaluation of ELISA based on the recombinant OmpL1 or LipL41 antigens of Leptospira interrogans serovar autumnalisAnnals of Tropical Medicine & Parasitology,2008, 102:  699-708.

  29. Vijayachari P, Sugunan A.P, Sharma S, Roy S, Natarajaseenivasan K, Sehgal S.C.  Leptospirosis in the Andaman Islands, India.  Trans. Royal Society of Trop. Med. Hyg., 2008, 102: 117-22.

  30. Prabhu N, Innocent JP, Chinnaswamy P, Natarajaseenivasan K, Sarayu L.   In vitro evaluation of Eclipta alba against serogroups of Leptospira interrogansIndian J of Pharmaceutical Sciences, 2008, 70:  788-91. 

  31. Shanmughapriya S, Krishnaveni J, Selvin J, Gandhimathi R, Arunkumar M,  Thangavelu T,  Seghal Kiran G,  Natarajaseenivasan K. Optimization of extracellular thermotolerant alkaline protease produced by marine Roseobacter sp(MMD040). Bioprocess and Biosystems Engineering, 2008, 31:427–433.

  32. Seghal Kiran G, Shanmughapriya S, Jayalakshmi J, Selvin J, Gandhimathi R, Sivaramakrishnan S, Arunkumar M, Thangavelu T, Natarajaseenivasan K. Optimization of extracellular psychrophilic alkaline lipase produced by marine Pseudomonas sp. (MSI057). Bioprocess and Biosystems Engineering, 2008, 31:483–492.

  33. Shanmughapriya S, Aseer Manilal,  Sujith S, Joseph Selvin,  Seghal Kiran G,  Natarajaseenivasan K. Antimicrobial activity of seaweeds extracts against multiresistant pathogens. Annals of Microbiology, 2008, 58 (3): 169-175.

  34. Gandhimathi, R,  Arunkumar M, Joseph Selvin,  Thangavelu T,  Sivaramakrishnan S,  Seghal Kiran G,  Shanmugha Priya S,  Natarajaseenivasan K. Antimicrobial potential of sponge associated marine actinomycetes. Journal of Medical Mycology, 2008, 18: 16-22.

  35. Radhika Krishna, Maripandi A, Suresh SSSR, Ponmurugan K. Natarajaseenivasan K.  Characterization of leptospiral isolates by using PCR-Restriction Fragment Length Polymorphism analysis.  Advanced Biotech, 2008,  12-16.

  36. Subashkumar, R., Vivekanandhan G, Suresh S.S.R,  Natarajaseenivasan K,  Thayumanavan Tha,  Lakshmanaperumalsamy P. Typing of Aeromonas hydrophila  of fish and human diarrhoeal origin by outer membrane proteins and lipopolysaccharides. Indian Journal of Microbiology , 2007, 47:46-50.

  37. Sameer Sharma, Paluru Vijayachari, Attayoor P.S,  Natarajaseenivasan K, Manoj V. M,  Subhash C. S.  Seroprevalence of Leptospriosis among Jarwas-a Hunter-gatherer Primitive Negrito Tribe of Andaman and Nicrobar Islands, India.  Research Journal of Microbiology,  2006, 1:  95-100.

  38. Sameer Sharma, Paluru Vijayachari, Attayoor P. S,  Natarajaseenivasan K,  Subhash C. S.  Seroprevalence of Leptospriosis among high-risk population of Andaman Islands, India.  American Journal of Tropical Medicine and Hygiene,  2006, 74:  278-83

  39.  Natarajaseenivasan K,  Vijayachari P,  Sharma S,  Sugunan A.P,   Sehgal S.C. Phenotypic and genotypic conservation of OmpL1 and LipL41 among leptospiral isolates of Andaman Islands. Indian Journal of Medical Research, 2005, 122:  343-347.

  40.  Biswas D,  Roy S,  Vijayachari P,  Sugunan A.P,  Natarajaseenivasan K,  Sehgal S.C.  Comparison of immunoreactive proteins of commonly circulating serogroups of Leptospira in Andaman Islands, India.  Indian Journal of Medical Research, 2005, 121: 151-158.

  41.  Natarajaseenivasan K,  Vijayachari P,  Sharma S,  Roy S,  Sugunan A.P,  Biswas D, Sehgal S.C.  Phylogenetic relatedness among leptospiral strains belonging to same serovar recovered from patients with different clinical syndromes.  Infection, Genetics and Evolution, 2005, 5: 185-91.  

  42. Sugunan A.P,  Natarajaseenivasan K,  Vijayachari P, Sehgal S.C.  Percutaneous Exposure Resulting in Laboratory Acquired Leptospirosis – A Case Report.  Journal of  Medical Microbiology, 2004, 53: 1259-6253.

  43. Natarajaseenivasan K,    Prabhu N,   Selvanayaki K,   Raja S.S.S and   Ratnam S.  Human Leptospirosis in Erode, South India:  Serology, Isolation and Characterization of the isolates by Randomly amplified polymorphic DNA (RAPD) fingerprinting.  Japanese Journal of Infectious Diseases, 2004, 57:  193-197.

  44.  Natarajaseenivasan K,  Vijayachari P,  Sugunan  A.P, Sharma S,  Sehgal S.C.  Leptospiral proteins expressed during Acute and Convalescent phases of human Leptospirosis.  Indian Journal of Medical Research, 2004, 120: 151-159.

  45. Vijayachari P, Hartskeerl R.A,  Sharma S,  Natarajaseenivasan K,  Roy S,  Terpstra W.J Sehgal S.C.  A unique strain of leptospira isolated from a patient with pulmonary haemorrhages in Andaman islands: a proposal of serovar Portblairi of serogroup Sehgali.  Epidemiology and Infection, 2004, 132: 663-673. 

  46. Natarajaseenivasan K,   Raja S.S.S,    Ratnam S,   Rajendran N.R.  Evaluation of the cross reacting leptospiral serovars by MAT, ELISA and MSAT in rabbit hyper immune sera.  Indian Journal of Animal Sciences,  2003, 73:  957-959.

  47. Natarajaseenivasan K,   Boopalan M,   Selvanayaki K,   Suresh S.R,   Ratnam S.  Leptospirosis among Rice Mill Workers of Salem, South India.  Japanese Journal of Infectious Diseases,  2002, 55: 170-173.

  48. Natarajaseenivasan K,  Ratnam S.  Relative Superiority of IgM+IgG combined conjugate plate ELISA for detection of anti leptospiral antibodies among different occupational groups. Indian Journal of Animal Sciences, 2001, 71: 42-44.

  49. Natarajaseenivasan K,  Ratnam S.  Recent leptospira   isolates from Tamilnadu.  Indian Journal of Animal Sciences,  2000, 70: 551-555.

  50. Biswal B.C, Kar B.C ,Ratnam S, Sureshbabu L,  Natarajaseenivasan K. Evidence of Antibodies to Leptospira among Farm cattle and Farm workers in Orissa State. Indian Veterinary Journa,  2000, 77: 622-623.

  51. Natarajaseenivasan K,  Ratnam S.  Isolation of  Leptospira javanica  from sheep.  Indian Journal of Animal Sciences, 1999, 69: 759-761.                                                  

  52. Ratnam S, Natarajaseenivasan K.  Possible hospital transmission of leptospiral InfectionJournal of Communicable Diseases,  1998, 30: 54-56.

  53. Natarajaseenivasan K,  Ratnam S. Seroprevalence of  Leptospiral infection in an Agricultural based village in Tamilnadu.  Cheiron, 1997, 26: 5-6.

  54. Natarajaseenivasan K,    Ratnam S. A probable   virulence  factor of javanica isolates.  Indian Journal of Animal Sciences,  1997, 68: 139-140.

  55. Ratnam S,  Sureshbabu L,  Natarajaseenivasan K.  Dynamism of MAT and type Specific IgM and IgG ELISA titres in leptospirosis cases.  Biomedicine,  1997, 17: 21-24.

  56. Natarajaseenivasan  K, Ratnam S.    Prevalence of   Leptospirosis in a cattle farm.  Indian Journal of Animal Sciences, 1997, 67: 860-861.

  57. Natarajaseenivasan K,  Ratnam S.  Experimental  leptospirosis in laboratory mice and  rats.  Journal of Communicable Diseases,  1997, 29: 291-293.

  58. Natarajaseenivasan K,  Ratnam S.  A comparison of MAT and ELISA among clinically suspected Leptospirosis cases.  Biomedicine,  1996, 16: 29-31.

  59. Sureshbabu,  Ratnam S,  Natarajaseenivasan K,  Helen Manual P.S, Sunithi Solomon, Padmanaban V.D.  Standardization of Dipstick ELISA in Leptospiral antigen detection.  Cheiron,  1996, 25: 93-98.

  60. Natarajaseenivasan K,  Ratnam S.  An investigation of Leptospirosis in a laboratory animal house.  Journal of Communicable Diseases,  1996, 28: 153-157.

  61. Ratnam S,  Natarajaseenivasan K.  Leptospiral illness in calves.  Indian Journal of Animal Sciences, 1996, 66: 32-33.

  62. Natarajaseenivasan K,  Ratnam S,  Ramadass P,  Helen Manual P.S.  Persistence of dinger’s rings by Leptospira interrogans serovar australis in semi solid EMJH medium.  Indian Veterinary Journal,  1996, 73: 571-572.

  63. Rathinam Shivakumar, Ratnam S, Suresh Babu L, Natarajaseenivasan K.  Leptospiral antibodies in patients with recurrent ophthalmic involvement.  Indian Journal of Medical Research,  1996, 103: 66-68.

  64. Vanaja R,  Ratnam S,  Suresh Babu L,  Natarajaseenivasan K,  Vimala Jepegnanam.  Biochemical changes in Experimental and Clinical Leptospirosis. Biomedicine, 1995, 15: 45-48.

  65. Suresh B,  Murthi V.A,  Suresh Babu L,  Natarajaseenivasan K,  Ratnam S.  Frog as carrier of leptospires.  Indian Journal of Animal Sciences,  1994, 64: 736-738.

  66. Suresh Babu, Natarajaseenivasan K,  Muthusethupathy M.A,  Shiva Kumar S,  Jayakumar M,  Helen Manual P.S, Sunithi Solomon,  Suresh B,  Ratnam S.  Survival of leptospires in different pH.  Indian Journal of Medical Microbiology,  1994, 12: 44-45.

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Maxwell H.Gluck Equine Research Center
Department of Veterinary Science, University of Kentucky
Lexington, Kentucky 40546-0099

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