University of Kentucky College of Agriculture, Food & Environment

 

Gluck Center > Directory > Gluck Faculty >Issel, CJ > Curriculum Vitae

CURRICULUM VITAE

Charles J. Issel, DVM, PhD
cissel@uky.edu
(859) 218-1096

Professor
Department of Veterinary Science
Wright-Markey Chair of Equine Infectious Diseases
M.H. Gluck Equine Research Center
College of Agriculture, Food & Environment
University of Kentucky

Academic training

University of California, Berkeley; A.B., 1965. (Zoology)

University of California, Davis; D.V.M., 1969.

University of Wisconsin, Madison; M.S., Ph.D., 1971, 1973. (Veterinary Science)

Positions Held:

June, 1990 to present --
Wright-Markey Chair of Equine Infectious Diseases, University of Kentucky

August, 1974 to 1990 --
Assistant Professor to Professor of Veterinary Virology and Veterinary Science, Louisiana State University

August, 1973 - August, 1974
Veterinary Medical Officer, USDA, Plum Island Animal Disease Center

Grant support

Industry: Vaccine design and evaluation, Diagnostics (continuous support 15 years)

NIH: Molecular virology, vaccine evaluation (continuous support since 1988; collaboration with Dr. RC Montelaro at the University of Pittsburgh).

Patents

6461616 EIAV P26 Deletion Vaccine and Diagnostic, US Patent Issued October 8, 2002.

6528250 Equine Infectious Anemia Challenge Model For Testing Vaccines, Diagnostics and Treatments, US Patent Issued March 4, 2003.

01966602.3-2402-US0127599 EIAV Chimeric Vaccine and Diagnostic, European Patent Issued April 24, 2003.

6585978 EIA Vaccine and Diagnostic, US Patent Issued July 1, 2003.

6727078 EIAV P26 Deletion Vaccine and Diagnostic, US Patent Issued April 27, 2004.

7026113 EIA Vaccine and Diagnostic, US Patent issued April 11, 2006. 

Selected Publications

Li, F., Leroux, C., Craigo, J.K., Cook, S.J., Issel, C.J., and Montelaro, R.C. 2000. The S2 gene of equine infectious anemia virus is a highly conserved determinant of viral replication and virulence properties in experimentally infected ponies. J. Virol. 74:573-579.

Hammond, S.A., Li, F., McKeon, B.M., Sr., Cook, S.J., Issel, C.J., and Montelaro, R.C. 2000. Immune responses and viral replication in long-term inapparent carrier ponies inoculated with equine infectious anemia virus. J. Virology 74:5968-5981.

Cook, S.J., Cook, R.F., Montelaro, R.C., and Issel, C.J. 2001. Differential responses of Equus caballus and Equus asinus to infection with 2 pathogenic strains of equine infectious anemia virus. Vet Micro. 79:93-109.

Leroux, C., Craigo, J.K., Issel, C.J., and Montelaro, R.C. 2001. Equine infectious anemia virus genomic evolution in progressor and non-progressor ponies. J. Virol. 75:4570-4583.

Issel, C.J., Sinclear, J.N., Bolin, P.S., Cook, S.J., Anderson, G., Marshall, M., Rogers, L.E., Mathis, J., and Lekas, J. 2000. Investigations on equine infectious anemia in equids from the Uintah Basin in Utah, 1999-2000. US An Health Assn. Proc. 104:372-382.

Craigo, J.K., Leroux, C., Howe, L., Steckbeck, J.D., Cook, S.J., Issel, C.J., and Montelaro, R.C. 2002. Transient immune suppression of inapparent carrier ponies infected with neutralization-resistant EIAV redirects antibody responses and lowers steady state viral replication. J. Gen Virol 83:1353-1359.

Zhou, W., Cook, R.F., Cook, S.J., Hammond, S.A., Rushlow, K., Ghabrial, N.N., Berger, S.L., Montelaro, R.C., and Issel, C.J. 2002. Multiple RNA splicing and the presence of cryptic RNA splice donor and acceptor sites may contribute to low expression levels and poor immunogenicity of potential DNA vaccines containing the env gene of equine infectious anemia virus (EIAV). Vet Micro 88/2:127-151.

Cook, R.F., Cook, S.J., Li, F., Montelaro, R.C., and Issel, C.J. 2002. Development of a multiplex real-time reverse transcriptase-polymerase chain reaction for equine infectious anemia virus (EIAV). J Virol Meth 105:171-179.

Howe, L., Leroux, C., Issel, C.J., and Montelaro, R.C. 2002. Equine infectious anemia virus envelope evolution in vivo during persistent infection progressively increases resistance to in vitro serum antibody neutralization as a dominant phenotype. J. Virol. 76(21):10588-10597.

Li, F., Craigo, J.K., Howe, L., Steckbeck, J.D., Cook, S., Issel, C., and Montelaro, R.C. 2003. A live attenuated EIAV proviral vaccine with a modified S2 gene provides protection from detectable infection by intravenous virulent virus challenge of experimentally inoculated horses. J. Virology 77(13):7244-7253.

Reis, J.K.P., Craigo, J.K., Cook, S.J., Issel, C.J., and Montelaro, R.C. 2003. Characterization of EIAV LTR variability and compartmentalization in various reservoir tissues of long term inapparent carrier ponies. Virology 311:169-180.

Cook, R.F., Cook, S.J., Berger, S.L., Leroux, C., Ghabrial, N.N., Gantz, M., Bolin, P.S., Mousel, M.R., Montelaro, R.C. and Issel, C.J. 2003. Enhancement of equine infectious anemia virus virulence by identification and removal of suboptimal nucleotides. Virology 313:588-603.

Jin, S., Issel, C.J., and Montelaro, R.C. 2004. Differential serological diagnosis of equine infectious anemia virus infected and vaccinated horses using recombinant S2 protein. Clin. and Diag. Lab. Imm. 11(6):1120-1129.

Issel, C.J., and Cook, S.J. 2004. Equine infectious anemia testing and control of EIA: how much is enough? USAnHealthAssn Proc 108:316-327.

Howe, L., Craigo, J., Issel, C.J., and Montelaro, R.C. 2005. Development of serum neutralizing antibodies to a neutralization resistant EIAV?PND envelope variant after immune suppression of experimentally infected ponies results from changes in host antibodies directed to gp90 V3 and V4 domains. J. Gen. Virol. 86:139-149.

Craigo, J.K., Steckbeck, J.D., Durkin, S., Howe, L., Cook, S.J., Issel, C. and Montelaro, R.C. 2005. Discerning an effective balance between equine infectious anemia virus attenuation and vaccine efficacy. J. Virol. 79(5):2666-2677.

Issel, C.J. 2005. Equine infectious anemia (EIA): 25 years and $600,000,000 later. Equine Disease Quarterly. Newsletter: 14(1).

Craigo, J.K., Sturgeon, T., Cook, S.J., Issel, C.J., Leroux, C. and Montelaro, R.C. 2005. Apparent elimination of EIAV ancestral species in a long-term inapparent carrier. Virology.(October issue)

Cook, R.F., Cook, S.J., Bolin, P.S., Howe, L.J., Montelaro, R.C. and Issel, C.J. 2005. Genetic immunization with codon-optimized equine infectious anemia virus (EIAV) surface unit (SU) envelope protein gene sequences stimulates immune responses in ponies. Vet Microbiol. 108(1-2):23-37.

Craigo, J.K., Sturgeon, T., Cook, S.J., Issel, C.J., Leroux, C. and Montelaro, R.C.  2006.  Apparent elimination of EIAV ancestral species in a long-term inapparent carrier.  Virology 344:340-353.

Craigo, J.K., Durkin S., Sturgeon TJ, Tagmyer, T., Cook SJ, Issel CJ, and Montelaro RC.  2006.  Immune suppression of challenged vaccinates as a rigorous assessment of sterile protection by lentiviral vaccines.  Vaccine 2006:834-845.

Tagmyer, T., Craigo, J.K., Cook, S.J., Issel, C.J. and Montelaro, R.C.  2007.  Characterization of broadly reactive T-helper and CTL epitopes in the envelope proteins of equine infectious anemia virus.  J. Gen Virology 88:1324-1336. 

Issel, C.J., Cordes, T.R., and Halstead, S.  2007. Control of EIA should take some new directions. Article#8787 on TheHorse.com (accessed 2/28/2007 at  http://www.thehorse.com/ViewArticle.aspx?ID=8787).

Issel, C.J. and Anderson, G.  2007.  The stigma of EIA is misplaced.  Article#9038 on TheHorse.com (accessed 3/2/2007 at http://www.thehorse.com/ViewArticle.aspx?ID=9038).

Craigo JK, Zhang B, Barnes S, Tagmyer TL, Cook SJ, Issel CJ, Montelaro RC.  2007.   Envelope variation as a primary determinant of lentiviral vaccine efficacy.  Proc Nat Acad Sci USA 104: 15105-15110.

 

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